Coding

Part:BBa_K283007:Design

Designed by: Liu Chenli   Group: iGEM09_HKU-HKBU   (2009-10-20)

sp-eGFP-streptavidin-AIDAc


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal BamHI site found at 17
    Illegal BamHI site found at 949
    Illegal BamHI site found at 2573
    Illegal XhoI site found at 2779
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 181
    Illegal EcoRI site found at 1675
    Illegal EcoRI site found at 2371
    Illegal PstI site found at 1723
    Illegal PstI site found at 2309
    Illegal AgeI site found at 991
    Illegal AgeI site found at 1042
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Reference:Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of Bacteriology, 2006vol.188(no.13)

Source

Pathogenic Escherichia coli strain 2787 (O126:H27) The genes encoding AIDA (adhesin involved in diffuse adherence) are located on two open reading frames, orfA and orfB. The latter codes for the AIDA autotransporter system, which is synthesized as a pre-pro-protein that is C-terminally processed to generate the mature adhesin AIDA-I and the outer membrane integrated AIDAC, which functions as the translocator for AIDA-I. orfA codes for a 45-kDa cytoplasmic protein which is required to modify AIDA-I such that it adheres to target cells. Although the AIDA system has first been characterized in a clinical E. coli isolate responsible for infant diarrhea, AIDA genes have been detected only in about 2 to 4% of human isolates . Thus, an animal reservoir for AIDA may exist.

References

Polar localization of the autotransporter family of large bacterial virulence proteins.Journal of Bacteriology, 2006vol.188(no.13)